Genetic diversity and relationships in mulberry genus. The cultivars with multiple flower forms, which makes it difficult to make judgment by means of a flowerformbased classification system, have been significantly characterized using leaf morphology or issr markers. Nonanchored inter simple sequence repeat issr markers. Fang 1the nurturing station for the state key laboratory of subtropical silviculture, zhejiang agriculture and forestry university, zhejiang, china. Finally, our findings will help for breeding and conservation of kongpo monkshood. A total of 219 issr clear and reproducible fragments were generated with issr primers in. Issr markers for genetic diversity studies in trifolium species. Generally, all the issr markers used in the study produced clear and reproducible amplification profiles.
Development and characterization of speciesdiagnostic issr. Using rapd and issr molecular markers to analyze the genetic. Walp cowpea accessions using informative molecular markers is imperative for their genetic improvement and conservation. New lecture material about issr marker basic information and things to.
Aug 08, 2019 each primer generated at least one speciesdiagnostic issr marker for a total of six. A similar number of private and marker bands was generated by both markers in all the species examined and one discriminant issr frag ment was obtained for. Inter ssr issr fingerprinting was developed such that no sequence knowledge was required. Cluster analysis using upgma by rapd and issr markers obviously revealed distinct diversity between genotypes.
Issr primers amplify dna segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Issrmarkers assisted genetic diversity assessment of. Comparative study of the marker systems based on pic, emr and mi values revealed scot markers to be more informative than other markers. Pdf rapd and issr markers for molecular characterization. Genetic diversity and relationships in mulberry genus morus.
Assessment of genetic diversity in vigna unguiculata l. Genetic stability using rapd and issr markers in efficiently in vitro regenerated plants of inula royleana dc. Application of inter simple sequence repeat issr markers to. Unlimited viewing of the articlechapter pdf and any associated supplements and figures. Issr marker would be more suitable for analyzing the distinct genetic differences to select special individuals, and irap for studying population genetic diversity or. Using issr markers, we efficiently and successfully detected genetic variation among kongpo monkshood individuals. Greater polymorphism was detected by aflps when compared with rapd and issr analyses using the same germplasm array rapd 36.
Rapd, issr, ipbs and scot markers were employed to study the genetic relationship of 50 different genotypes belonging to 5 native bamboos of northeast india. The issr and ipbs markers also produced average pic values of 0. Issr markers have numerous advantages that make them easy to be applied in several crop studies. Comparison of issr and aflp markers in assessing genetic diversity among nettle urtica dioica l. Inter simple sequence repeat issr were used to evaluate the genetic diversity of kongpo monkshood aconitum kongboense l. Genetic variation and clonal diversity of psammochloa villosa. The average marker index mi values derived from the three different marker systems differed dramatically. Rapd random amplified polymorphic dna and issr inter simple sequence repeats markers were used to study dna polymorphism of thirty grewia optiva genotypes raised from seeds collected from various districts of himachal pradesh india and selected. Author links open overlay panel samar amin a tareq a. Molecular diversity assessment in chickpea through rapd and. Assessment of genetic diversity of vigna unguiculata l. Inter simple sequence repeat issr markers for assessment of genetic polymorphism and phylogenetic relationships of the silkworm bombyx mori l. A comparative analysis of issr and rapd markers for study of genetic diversity in shisham dalbergia sissoo. The application of issr markers in genetic variance detection.
Genetic diversity analysis of walnut juglans regia l. Inter simple sequence repeat issrpcr is a technique, which involvesthe use of microsatellite sequences as primers in a polymerase chainreaction to generate multilocus markers. Rapd and issr fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut arachis hypogaea cultivars and wild speciess n raina, v. Issr markers were distributed throughout the chromosomes. Culley dominant markers, such as rapds and issrs, have become popular in molecular studies in recent years. Genetic differences among luotiantianshi diospyros kaki. A genomic dna clone representing 1aminocyclopropane1carboxylic acid acc synthase gene, involved in ethylene biosynthesis, was also mapped. Pdf molecular characterization of cultivated bromeliad. Walnut juglans regia is one of the most essential nutritive and economic nut crops. Although 44 issr fragments and 29 rapd fragments statistically showed a 3. From 70 accessions of three populations, 10 out of 100 informative issr primers were chosen for polymorphism analysis. Identification of issr markers associated with productivity traits in silkworm, bombyx mori l.
The dendrogram indicated that the issr markers succeeded in distinguishing most of the 20 varieties in relation to their genetic background and geographical origin. Rapd and issr molecular markers were used for this genetic diversity study. Singlelocus ssr markers have been developed for a number of species, although there is a major bottleneck in developing ssr markers whereby flanking sequences must be known to design 5anchors for polymerase chain reaction pcr primers. Species delimitation and genetic diversity analysis in salvia.
Pdf genetic diversity based on autogeny and issr markers. It is concluded that issr technique provides a quick, reliable and highly informative system for dna fingerprinting. Inter simple sequence repeat issr markers for assessment. Clonal diversity in the rare calamagrostis porteri ssp. Issr markers as a tool for the assessment of genetic diversity in passiflora article pdf available in biochemical genetics 4978. Description issrs are dna fragments of about 1003000 bp located between adjacent, oppositely oriented microsatellite regions. Using rapd and issr molecular markers to analyze the. Genetic similarities among wheat lines based on issr and rapd markers ranged from 0. Pdf intersimple sequence repeats issrs are regions in the genome flanked by microsatellite sequences. View the article pdf and any associated supplements and figures for a period of 48 hours. Degree of polymorphism was found relatively higher in bruguiera gymnorrhiza 26. Genetic differences among luotiantianshi diospyros. Species delimitation and genetic diversity analysis in.
Out of the 100 issr primers and 100 srap primer combinations. In this study, intersimple sequence repeats issr markers were applied to assess genetic diversity and genetic relationships of 14 cultivars of durian durio. They provide highly discriminating information with good reproducibility, and are relatively abundant. Few molecular studies have been performed on this species. Besides, classic and biotechnical techniques have been efficiently used in plant breeding programs and dna markers are ordinarily used to allow cultivar.
Download pdf abstractexcerpt full text pdf contributor notes intersimple sequence repeat issr markers were used to evaluate the effects of in vitro culture on genetic variation in habanero pepper capsicum chinense jacq. It is a simple and quick methodthat combines most of the advantages of microsatellites ssrs andamplified fragment length polymorphism aflp to the universality ofrandom amplified polymorphic dna. Development and characterization of speciesdiagnostic issr and scar dna markers for differentiating red maple acer rubrum and silver maple a. Molecular identification of sex in phoenix dactylifera. Issr markers are highlypolymorphic and are useful in studies on genetic diversity, phylogeny, genetagging, genome. For issr markers, a total of 10 primers consisting of di and trirepeat motifs were used for initial screening with 3 specimens. Crossbreeding of phyllostachys species poaceae and identification of their hybrids using issr markers x. Issrmarkers assisted genetic diversity assessment of acid lime citrus aurantifolia. Crossbreeding of phyllostachys species poaceae and. Development and characterization of speciesdiagnostic. We predicted that luotiantianshi would be a cultivar group rather than a single cultivar.
Rapd and issr fingerprints as useful genetic markers for. Effectiveness of issr markers for determination of the. Issr markers present a precisely discriminatory power in tree peony cultivar classification without environmental influences. Pdf the application of issr markers in genetic variance. In the present study, morphological traits, issr intersimple sequence related and srap sequencerelated amplified polymorphism markers were used to analyze the genetic diversity of 59 s. This technique has been widely used in the studies of cultivar. Evaluation of genetic relationship among varieties of capsicum annuum l.
The use of issr markers to identify texas bluegrass interspecific hybrids. The use of issr and rapd markers for genetic diversity. Pdf issr markers as a tool for the assessment of genetic. Issr markers as a tool for the assessment of genetic. Inter simple sequence repeat issr pcr is a technique, which involvesthe use of microsatellite sequences as primers in a polymerase chainreaction to generate multilocus markers. Genetic variation and clonal diversity of psammochloa. Pdf comparison of issr and aflp markers in assessing. Molecular diversity assessment in chickpea through rapd and issr markers. Issr markers and their applications in plant genetics.
Efficiency of rapd, issr, ipbs, scot and phytochemical. Pdf inter simple sequence repeat issr markers for genetic. Issr and rapd molecular markers were used to analyze p. Genetic stability using rapd and issr markers in efficiently. Using rapd and issr molecular markers to analyze the genetic diversity of rose scented pelargonium populations qing. Applicability of leaf morphology and intersimple sequence. Issr markers for assessing dna polymorphism and genetic. Comparison of issr and rapd markers to characterize three. Issr markers are inherited in mendelin mode and segregated as dominant markers. Effectiveness of issr markers for determination of the aneura. Pdf genetic maps of rapd, aflp and issr markers in. Evaluation of genetic relationship among varieties of. Genetic diversity analysis of 96 walnut genotypes from kashmir was done using rapd and 19 issr markers. Molecular characterization of cultivated bromeliad accessions with intersimple sequence repeat issr markers.
Issr markers detected more diversity than rapd markers in three of the four populations examined. A total of 8, 77 and 144 bands were detected by 16 rapd primers, 5 issr primers and 23 srap primer combinations, among which 58. Three different dnabased techniques, random amplified polymorphic dna rapd, inter simple sequence repeat issr and amplified fragment length polymorphism aflp markers, were used for fingerprinting dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. Download fulltext pdf download fulltext pdf download fulltext pdf download fulltext pdf the application of issr markers in genetic variance detection among durian durio zibethinus murr. Identification of issr markers associated with productivity.
Wani a zahoor ahmad kaloo a seema singh a riffat john b umer majeed b gowhar. Highlights we analyzed the genetic differences among 43 luotiantianshi genotypes native to china using issr and irap markers. The present study showed that issr molecular markers could be used in salvia species delimitation along with morphological study. Identification of issr and rapd markers linked to yield traits in. Evaluation of genetic diversity in lentinula edodes. In the present paper, the issr technique was employed to study genetic diversity in italian a. Research stream flow projection for muar river in malaysia using precis hechms model. Population genetic analysis of issr data theresa m.
Random amplified polymorphic dna rapd, intersimple sequence repeat issr and sequencerelated amplified polymorphism srap markers were used to evaluate the genetic diversity among 23 elite lentinula edodes strains in china. If the inline pdf is not rendering correctly, you can download the pdf. Shivakumar bakkappa1 and subramanya gopal1 1department of studies in sericulture, university of mysore, manasagangothri, mysore570006, india. The use of issr and rapd markers for genetic diversity among. Issr markers for assessing dna polymorphism and genetic characterization of cattle. Inter simple sequence repeat issr polymorphism and its. Pdf intersimple sequence repeat issr markers to study. Comparison of rapd, issr, and aflp molecular markers to. Random amplified polymorphic dna rapd and intersimple sequence repeat issr were assayed to determine the genetic diversity of 80 barley specimens. Analysis of genetic diversity among wild and cultivated chickpea genotypes employing issr and rapd markers abstract fulltext html download as pdf. Use of efficacious molecular markers to obtain the required knowledge of the genetic diversity within the local and regional germplasm collections can enhance the overall effectiveness of cowpea improvement. Apr 12, 2016 issr markers comprise a few highly informative multiallelic loci.
Structure and reticulation analyses revealed some degree of gene fl ow among the species. Genetic linkage map of issr and rapd markers in einkorn. W qian, s ge, dy honggenetic variation within and among populations of a wild rice oryza granulata from china detected by rapd and issr markers theoretical and applied genetics, 102 2001, pp. Genetic diversity assessment of a germplasm collection of. The utilization of molecular markers at this stage for early and rapid identification of sex is important due to the lack of morphological markers. Effectiveness of issr markers for determination of the aneura pinguis cryptic species and aneura maxima. There are several reports on the efficacy of pcrbased techniques including issr among other markers in evaluating the relationship or variability between different varieties of capsicum ibarratorres et al. Pdf nonanchored inter simple sequence repeat issr markers. Analysis of genetic diversity among wild and cultivated. Evaluation of genetic diversity among kongpo monkshood. A testcross population previously used for mapping of rapd and issr markers was used in this study. Out of these, 7 primers gave no amplification at all, while only 3 primers were found to give clear and polymorphic patterns and were subsequently used to analyze the entire set of 80 genotypes. Pdf a comparative analysis of issr and rapd markers for. It is a simple and quick methodthat combines most of the advantages of microsatellites ssrs andamplified fragment length polymorphism aflp to the universality ofrandom amplified polymorphic dna rapd.
In the future, the markers used in this study, in combination with other molecular techniques, could provide a useful panel of issr markers for largescale dna fingerprinting of poplar cultivars and determination of the genetic relationships among these cultivars. Observation of higher pic values for scot and issr markers as compared to the pic values of rapd was earlier reported during the genetic diversity studies of several plants noormohammadi et al. Effectiveness of issr markers for determination of the aneura pinguis cryptic species and. Efficiency of using rapd and issr markers in evaluation of. Rapd markers in general are dominant thereby they have lower information content than codominant markers in the linkage analysis of f2 populations alizadeh et al.
Genetic diversity based on autogeny and issr markers in culex pipiens mosquitoes. The mantel test did not show a correlation between genetic distance and the geographical distance of the studied populations. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. In this study, rapd assays produced 97 bands, of which 40 were. Grapevine cultivars originating in central and eastern europe and north america using issr markers download download pdf. Application of issr markers to fingerprinting of elite. Salvia miltiorrhiza is one of the most important traditional chinese medicinal plants for its therapeutic effects. Nkongolo a b a department of biology, laurentian university, sudbury, on p3e 2c6, canada. Authors contributions this work was carried out by the. Issr markers are useful in detecting genetic polymorphisms among accessions by generating a large number of markers that target multiple microsatellite loci distributed across the genome.
It is known for its fine flavour and could represent an important resource for cultivation programs in desert areas. Of these three techniques, while aflp is the most labour intensive and timeconsuming, it is also the most reliable. Issrs are amplified by pcr using microsatellite core sequences as primers with a few selective nucleotides as anchors into the nonrepeat adjacent regions 1618 bp. A comparative analysis of issr and rapd markers for studying genetic diversity in iranian pistachio cultivars ayda tagizad1, jafar ahmadi 2, rahim haddad1 and mahdi zarrabi3 1 agricultural biotechnology department, faculty of engineering and technology, imam khomeini international university, qazvin, iran. Application of inter simple sequence repeat issr markers. The linkage map contains 360 dna markers distributed on 19 linkage groups, and covers a genetic distance of 1976 cm with an average distance of 5. Rapd and issr marker mediated genetic polymorphism of two. As an alternative to isozymes, these types of markers are especially attractive given their hypervariable nature, the vast numbers of loci that can be examined, and the small.
Random amplified polymorphic dna rapd and intersimple sequence repeat issr markers were used for comparative analysis of genetic variation in 42 sugar beet accessions. Issr markers are useful techniques for genetic diversity. A total of 24 polymorphic primers 12 rapd and 12 issr were used. Molecular diversity assessment in chickpea through rapd. Thus, nonanchored issr markers are a good choice for dna fingerprinting. Issr markers, which show dominant inheritance, use ssr repeatanchored primers and are being used as an alternate tool in diversity studies. Issr markers show differentiation among italian populations. Intersimple sequence repeat issr markers to study genetic diversity among cotton cultivars in associated with salt tolerance. Early sex identification of date palm phoenix dactylifera l. An extended linkage map for watermelon based on srap, aflp.
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